IMAGE

Fig. 4.

ID
ZDB-IMAGE-210708-47
Genes
Source
Figures for Sidik et al., 2021
Image
Figure Caption

Fig. 4.

Ethanol indirectly attenuates Shh signaling. a Alcian blue and Alizarin red whole mount staining of 4 dpf control, cyclopamine-treated (50 μM), and ethanol- and cyclopamine-treated (1% ethanol plus 50 μM cyclopamine) wild-type embryos from 6 hpf to 4 dpf. Horizontal panels represent the spectrum of phenotypes observed for the treatment groups. Dorsal view, anterior to the left. b Quantification of the effect on inner lens-to-lens width. Both cyclopamine alone and cyclopamine and ethanol were significantly different from controls (p < 0.0001; Tukey’s honest test). Sample size and p values provided in Additional file 9: Table S2. c RT-qPCR of ptch2 in 10 hpf control and ethanol- and/or cyclopamine-treated wild-type embryos. n = 4, for all groups (p < 0.05; Tukey’s honest test). P values provided in Additional file 9: Table S2. d KEGG pathway schematic illustrating differential expression due to ethanol treatment in the sonic hedgehog (Shh) signaling pathway. Color coding indicates log2 fold differences due to ethanol treatment across all samples

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Biol.