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Fig. 2 (A–T) Fluorescence levels in zebrafish embryos upon incubation to 1 µm rhodamine B along with chemicals at different concentrations for 2 h. The percentage fluorescence relative to the respective control (0.1% DMSO) was plotted vs. chemical concentration. Symbols and bars represent means ± SE. Data points are from 2 to 6 replicates; each replicate is the mean fluorescence measured in eight embryos exposed and measured on the same day. Cyclosporin A (40 µm) was tested in each experimental series as positive control; exposures with rhodamine B along with 0.1% DMSO served as negative controls. Data were fitted with linear or nonlinear regression (HILL model, Eqn 1). For regression parameters, refer to Table 2.