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Figure 5 Effect of RD1 mutation in Mycobacterium marinum (Mm) on frequency of ocular infection. We infected four dpf larvae with 25 colony forming units (CFU) of RD1-mutant Mm. (A) Line graph showing significantly lower frequency of ocular infection with RD1 mutant Mm (compared with Figure 1A), even up to 10 dpi. The infected larvae, however, survived longer than during infection with wild-type (WT) Mm, as shown by the low slope of the graph. (B) Depletion of circulating monocytes with clodronate liposomes led to a drop in the ocular infection rate, the effect being opposite to that from infection with WT Mm.