PUBLICATION
Role of Extracellular Mycobacteria in Blood-Retinal Barrier Invasion in a Zebrafish Model of Ocular TB
- Authors
- Damera, S.K., Panigrahi, R.K., Mitra, S., Basu, S.
- ID
- ZDB-PUB-210407-41
- Date
- 2021
- Source
- Pathogens (Basel, Switzerland) 10(3): (Journal)
- Registered Authors
- Keywords
- dissemination, extracellular, macrophage, ocular, pathogenesis, tuberculosis, zebrafish
- MeSH Terms
- none
- PubMed
- 33805720 Full text @ Pathogens
Citation
Damera, S.K., Panigrahi, R.K., Mitra, S., Basu, S. (2021) Role of Extracellular Mycobacteria in Blood-Retinal Barrier Invasion in a Zebrafish Model of Ocular TB. Pathogens (Basel, Switzerland). 10(3):.
Abstract
Intraocular inflammation following mycobacterial dissemination to the eye is common in tuberculosis (TB)-endemic countries. However, the early host-pathogen interactions during ocular dissemination are unknown. In this study, we investigated the early events during mycobacterial invasion of the blood-retinal barriers (BRBs) with fluorescent-tagged Mycobacterium marinum (Mm), host macrophages, and retinal vasculature in a zebrafish model of ocular TB. We found that Mm invaded the vascular endothelium in either the extracellular or intracellular (inside phagocytes) state, typically 3-4 days post-injection (dpi). Extracellular Mm are phagocytosed in the retinal tissue and progress to form a compact granuloma around 6 dpi. Intracellular Mm crossing the BRBs are likely to be less virulent and either persist inside solitary macrophages (in most cases) or progress to loosely arranged granuloma (rarely). The early interactions between mycobacteria and host immune cells can thus determine the course of disease during mycobacterial dissemination to the eye.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping