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Fig. 2 Craniofacial and other morphological defects in tango1 mutants are accentuated by ctage5 deletion. (A) Representative lateral (left) and ventral (right) images of Alcian blue–stained 4dpf larvae. (B) Representative images of plastic-sectioned and toluidine blue–stained 4dpf larvae cropped to show craniofacial alterations. TC = trabeculae, BC = buccal cavity, CH = ceratohyal cartilage. (C) Representative maximum intensity projection images showing TUNEL staining of 4dpf lens and sclera. (D) Representative images of plastic-sectioned and toluidine blue–stained 4dpf lens. (E) Representative images of lens dissected from 3-mo-old zebrafish. (F) Representative images showing ventricle-enriched cardiac myosin light chain (CMLC:GFP) expression. (G) Representative brightfield image of the trunk for each genotype. Arrowheads detail blood pooling in the caudal vein.