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Figure 3—figure supplement 3. Typical defect size caused by phase vortices in simulation is consistent with the one observed in embryonic experimental data.

(A)-(E) Defect size distributions for different washout timing in embryonic experimental data. Washout at (A) twash-out = 9.5 hr post fertilization (hpf: 0 somite stage (ss); n = 28), (B) 11 hpf (3 ss; n = 22), (C) 12.5 hpf (6 ss; n = 28), 14 hpf (9 ss; n = 30) and 15.5 hpf (12 ss; n = 26). Defect size indicates how many consecutive segment boundaries are defective in between FRS and PLD. (F)-(G) Defect size distributions for different washout timing in simulation data. Washout at (F) twash-out = 0 ss, (G) 3 ss, (H) 6 ss, and (I) 9 ss. Simulation data for both left and right segments from 100 realizations in each washout timing are plotted. The model for the simulations includes the PSM shortening, change in cell advection pattern and increase in the coupling strength. The defect size was analyzed with the same simulation and experimental data shown in Figure 4 in the main text.

Acknowledgments
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