IMAGE

FIGURE 3

ID
ZDB-IMAGE-210324-13
Source
Figures for Liu et al., 2021
Image
Figure Caption

FIGURE 3

Gross morphology of zebrafish with itpr1b knocked down at 5-dpf. Lateral view of control MO-injected zebrafish embryos and embryos injected with itpr1b morpholino oligonucleotides (MO) (A–I). Calcium staining by calcein in MO-control, MO-itpr1-e4i4, and MO-itpr1-ATG morphants at 120 hpf (hours post-fertilization). Both MO-itpr1-e4i4, MO-itpr1-ATG morphants showed bimaxillary retrognathia [(A–C), red arrowed indicated the upper jaw, blue arrowed indicated the lower jaw]. The gut is filled with calcein (D–I,M–R). Fluorescent signals were apparent in 120-hpf embryos and restricted to the head skeleton (D–I,M–R). For MO-itpr1-e4i4 and MO-itpr1-e4i4 morphants, Meckel’s cartilage can barely be recognized [blue arrowhead, (E,F,H,I)]. From the ventral view (J–R), scoliosis can be observed in MO-itpr1-ATG (black arrowhead), Meckel’s cartilage is undistinguished (blue arrowhead). Fluorescent signals were greatly reduced in palatoquadrate and ethmoid and ectopterygoid. (S)itpr1b expression was induced in 72-hpf embryos, further increased at 96 hpf, and remained elevated at 120 hpf. (T) Graph presenting the quantification of the relative fluorescence intensity (RFI) of head skeleton bone mass (N = 10, ANOVA, ***P < 0.001). (U) Graph presenting the quantification of the inhibit rate (IR) of head skeleton bone mass (N = 10, ANOVA, ***P < 0.001). The region used to calculate bone mass is shown in panel (G) highlighted with the dashed rectangle. mc, Meckel’s cartilage; pq, palatoquadrate; ec, ectopterygoid; e, ethmoid.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Genet