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Fig. 1 Fig 1. Spontaneous activity in calcium imaging data. (A) Two-photon calcium imaging of the larval zebrafish optic tectum. NP, neuropil; PVL, periventricular layer. (B) Fluorescence traces from 10 example neurons. Dashed vertical lines indicate stimulus onset; colour represents azimuth angle of presented stimulus. (C) Example fluorescence trace segment illustrating that spontaneous calcium transients can occur just before stimulus onset, inflating stimulus-response estimates.
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