ZFIN Image: Dries et al., 2021, Figure 3

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Figure Caption

Figure 3

Ncam1b affects the expression of the Fgfr1a-target gene erm. Tg(ClaudinB::lynGFP) embryos were immunostained labeled for GFP (green). In situ hybridizations of probes detecting lef1 (Leading-Zone), fgfr1a, or erm (both in the Trailing-Zone) were visualized by NBT/BCIP staining. Primordia outlines are surrounded with a dotted line for better visibility. (A–C) Neither expression of lef1 nor of fgfr1a are affected in ncam1b-morphants, whereas erm expression is markedly reduced. (A′,C′) Neither ncam1a-morphants nor (A″,C″) siblings show alterations for lef1, fgfr1a, or erm. (D) Reduced erm expression after knockdown of ncam1b. Error bars show standard deviation. X²-test with ns = p ≥ 0.05. Scale bar 20 μm.

Acknowledgments

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