IMAGE

Fig. 5

ID

ZDB-IMAGE-210131-9

Genes

fosab
hbbe3

Publication

Facchinello et al., 2021 - Efficient clofilium tosylate-mediated rescue of POLG-related disease phenotypes in zebrafish

All Figures

Figures for Facchinello et al., 2021

Image

Figure Caption

Fig. 5 Altered signalling and mitochondrial dysfunction in <italic>polg</italic> mutants.

A The CREB signalling-targeted gene fosab is upregulated in 4 dpf polg^{sa9574/sa9574} mutants, compared to controls. B The Hif-Hypoxia signalling-targeted gene hbbe3 is upregulated in 4 dpf polg^sa9574/sa95 mutants, compared to controls. C–E Hif-Hypoxia signalling increases also in 6-dpf polg^ia302/ia302 mutants, as shown by the Hif-Hypoxia-responsive transgene HRE:mCherry. E Chart reporting the HRE fluorescence relative intensity (R.I.). F Diagram depicting the oxygen consumption rate (OCR) profile throughout Seahorse assay in 4 dpf wt and polg^{sa9574/sa9574} embryos under basal conditions, FCCP (p-triFluoromethoxyCarbonylCyanide Phenylhydrazone)-induced maximal respiratory capacity stimulation and ROT/AA (Rotenone/Antimycin A)-mediated inhibition. Three independent experiments were performed (N = 20). G Quantification of basal respiration in wt and polg^{sa9574/sa9574} mutants (N = 20, in triplicate).

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Death Dis.