Figure 3.

Figure 3.

(A) Overview of volume measurement for cell clusters. Representative cluster of cells from three dpf larvae mosaically expressing cytoplasmic mNeonGreen in basal cells (TgBAC(∆Np63:Gal4) embryos injected with UAS:mNeonGreen-P2A-mRuby3-CAAX plasmid at the 1 cell stage). (Left) Z-projection of a representative cell cluster before and 90 s after wounding. (Center) side view at the position indicated by the yellow line. (Right) cell height measured at each pixel. (B) Average volume over time for cell clusters exposed to different media, relative to their volume before wounding. n: number of cell clusters. N: number of larvae. Error bars are 95% bootstrapped confidence intervals of the mean. (C) (Left) Absolute volume measurements for different cell clusters before and 90 s after wounding. *p<0.05, two-tailed t-test on the average paired difference from each larva. See Table 2 for exact p-values and Cohen’s d. (Right) mean paired difference of the measurements shown at left. Distributions are kernel density estimates. (D–E) Average tissue speed (D) and cell cluster volume (E) over time for larvae treated with different concentrations of sodium chloride. 5 and 135 mM speed data are the same as in Figure 2A; 5 and 135 mM volume data are the same as in (B). (F) Average cell cluster volume 90 s after wounding relative to pre-wounding volume, plotted against the average 1^st principal component score for speed trajectories. Error bars are 95% bootstrapped confidence intervals of the mean. Linear regression of the Hypo and three NaCl conditions displayed with a dashed line (r² = 0.95).