PUBLICATION

Osmolarity-independent electrical cues guide rapid response to injury in zebrafish epidermis

Authors
Kennard, A.S., Theriot, J.A.
ID
ZDB-PUB-201124-4
Date
2020
Source
eLIFE   9: (Journal)
Registered Authors
Keywords
cell biology, developmental biology, zebrafish
MeSH Terms
  • Animals
  • Larva
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Gene Expression Regulation, Developmental/drug effects
  • Zebrafish
  • Sodium Chloride/chemistry
  • Sodium Chloride/pharmacology*
  • Epidermis/injuries*
  • Animals, Genetically Modified
  • Osmolar Concentration*
  • Plasmids
  • Electrophysiological Phenomena/physiology*
(all 13)
PubMed
33225997 Full text @ Elife
Abstract
The ability of epithelial tissues to heal after injury is essential for animal life, yet the mechanisms by which epithelial cells sense tissue damage are incompletely understood. In aquatic organisms such as zebrafish, osmotic shock following injury is believed to be an early and potent activator of a wound response. We find that, in addition to sensing osmolarity, basal skin cells in zebrafish larvae are also sensitive to changes in the particular ionic composition of their surroundings after wounding, specifically the concentration of sodium chloride in the immediate vicinity of the wound. This sodium chloride-specific wound detection mechanism is independent of cell swelling, and instead is suggestive of a mechanism by which cells sense changes in the transepithelial electrical potential generated by the transport of sodium and chloride ions across the skin. Consistent with this hypothesis, we show that electric fields directly applied within the skin are sufficient to initiate actin polarization and migration of basal cells in their native epithelial context in vivo, even overriding endogenous wound signaling. This suggests that, in order to mount a robust wound response, skin cells respond to both osmotic and electrical perturbations arising from tissue injury.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
la213TgTransgenic Insertion
    mu271TgTransgenic Insertion
      zf3374TgTransgenic Insertion
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        No data available
        Antibodies
        No data available
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        GAL4FFEFGGAL4FF
        GFPEFGGFP
        mAppleEFGmApple
        1 - 3 of 3
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        Mapping
        No data available
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        Citation
        Kennard, A.S., Theriot, J.A. (2020) Osmolarity-independent electrical cues guide rapid response to injury in zebrafish epidermis. eLIFE. 9:.