Figure 4

Figure 4

The cardiac hypertrophy biomarkers nppb and nppa are upregulated by ndufa7 depletion. (A) WT zebrafish embryos were injected with ndufa7 MO or control MO and then subjected to whole‐mount in situ hybridization with riboprobes against nppb. Scale bar, 75 μm. The experiments were performed in triplicate, with 20 individuals per condition. (B) cmlc2::GFP embryos were injected with ndufa7 MO or control MO at 1‐cell stage, and RNA was then extracted from harvested embryo hearts at 3 dpf or 4 dpf for RT‐qPCR. The experiments were performed in triplicate, with 60 individuals per condition. Statistical test: Student's t test. **P < .01 compared with control MO group, n = 6 measurements per condition. (C) nppb::F‐luc embryos were injected with ndufa7 splice‐blocking MO or control MO at 1‐cell stage and collected at 3 dpf and 4 dpf for the luciferase assay. The experiments were performed in triplicate, with n = 30 individuals per condition. Statistical test: Student's t test. **P < .01 compared with control MO group, n = 20 measurements per condition. (D) Wild‐type zebrafish embryos were injected with ndufa7 MO or control MO and then subjected to whole‐mount in situ hybridization with riboprobes against nppa probe. Scale bar, 75 μm. The experiments were performed in triplicate, with 20 individuals per condition. (E) cmlc2::GFP embryos were injected with ndufa7 MO or control MO at 1‐cell stage, and RNA was then extracted from harvested embryo hearts at 3 dpf or 4 dpf for RT‐qPCR. The experiments were performed in triplicate, with 60 individuals per condition. Statistical test: Student's t test. **P < .01, *P < .05 compared with control MO group, n = 6 measurements per condition