ZFIN ID: ZDB-IMAGE-200910-33
Figures for Chetty et al., 2020

Figure Caption/Comments:

Fig. 2 Overexpression of a constitutively active MAP2K1 construct compensates for a reduction in kdrl and kdr expression and partially rescues angiogenic defects in ets1−/−; etv2 MO embryos. (A) qPCR analysis of endothelial genes cdh5, kdrl and kdr in whole embryos at 32 hpf. Note the reduction in kdrl and kdr expression in ets1−/−; etv2−/−embryos compared to etv2−/−embryos (B-F″) Confocal images of 28 hpf Tg(kdrl:GFP) wildtype, ets1−/−, etv2 MO (0.25 ​ng), ets1−/−; etv2 MO (0.25 ​ng) and ets1−/−; etv2 MO; fli1aep:mCh-actMAP2K1 embryos. Note the mild angiogenic defects in etv2 MO (0.25 ​ng) embryos (D) and more severe defects in ets1−/−; etv2 MO embryos(E). (F-F″) Split images showing vascular GFP expression (F), mCherry expression from the actMAP2K1 construct (F′) and a merged image with GFP and mCherry (F″). (G) Quantification of ISVs in embryos from B-F. Note the increase in the number of ISVs in ets1−/−; etv2 MO; fli1aep:mCh-actMAP2K1 embryos compared to the ets1−/−; etv2 MO embryos. ∗P ​< ​0.05, ∗∗P ​< ​0.01; n.s – not significant; columns and horizontal bars in dotplots represent mean, error bars represent ​± ​SEM.

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