ZFIN Image: Chetty et al., 2020, Fig. 2

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Fig. 2 Overexpression of a constitutively active MAP2K1 construct compensates for a reduction in kdrl and kdr expression and partially rescues angiogenic defects in ets1−/−; etv2 MO embryos. (A) qPCR analysis of endothelial genes cdh5, kdrl and kdr in whole embryos at 32 hpf. Note the reduction in kdrl and kdr expression in ets1−/−; etv2−/−embryos compared to etv2−/−embryos (B-F″) Confocal images of 28 hpf Tg(kdrl:GFP) wildtype, ets1−/−, etv2 MO (0.25 ng), ets1−/−; etv2 MO (0.25 ng) and ets1−/−; etv2 MO; fli1aep:mCh-actMAP2K1 embryos. Note the mild angiogenic defects in etv2 MO (0.25 ng) embryos (D) and more severe defects in ets1−/−; etv2 MO embryos(E). (F-F″) Split images showing vascular GFP expression (F), mCherry expression from the actMAP2K1 construct (F′) and a merged image with GFP and mCherry (F″). (G) Quantification of ISVs in embryos from B-F. Note the increase in the number of ISVs in ets1−/−; etv2 MO; fli1aep:mCh-actMAP2K1 embryos compared to the ets1−/−; etv2 MO embryos. ∗P < 0.05, ∗∗P < 0.01; n.s – not significant; columns and horizontal bars in dotplots represent mean, error bars represent ± SEM.

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Acknowledgments

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Reprinted from Developmental Biology, 465(1), Chetty, S.C., Sumanas, S., Ets1 functions partially redundantly with Etv2 to promote embryonic vasculogenesis and angiogenesis in zebrafish, 11-22, Copyright (2020) with permission from Elsevier. Full text @ Dev. Biol.