ZFIN Image: Johansson et al., 2020, Figure 6

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Figure Caption

Figure 6

prl3 Mutants Are Rescued by a V-ATPase Inhibitor

(A) Single embryo transcriptomics of heterozygote Tg(β-actin:prl3a) zebrafish reveals atpv1aa is selectively downregulated versus wild-type control siblings. Adjusted p-value (p < 0.05) was determined by DESeq2 using the Wald test with Benjamini-Hochberg correction for multiple testing. See also Table S5.

(B) g:Profiler output shows V-ATPase complex GO enrichment for Tg(β-actin:prl3a) differentially expressed genes versus wild-type controls. Inset: diagram of V-type ATPase, all genes encoding components labeled red are downregulated in Tg(β-actin:prl3a) expressing embryos.

(C) K-nearest neighbor graph (SPRING webtool) of prl3a expression in neural crest lineages using Wagner et al. (2018) zebrafish 24 hpf single-cell data.

(D) GSEA plotting enrichment of V-ATPase genes in the prl3a-negative subpopulation of pigment cells from Wagner et al. (2018).

(E and F) (E) Images and (F) quantification of melanocytes in wild-type (WT) or prl3a⁻^/⁻ mutant embryo ± concanamycin A in a mitfa^vc7 regeneration assay, ANOVA using Tukey’s test, ^∗∗∗∗p < 0.0001.

Acknowledgments

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Reprinted from Developmental Cell, 54(3), Johansson, J.A., Marie, K.L., Lu, Y., Brombin, A., Santoriello, C., Zeng, Z., Zich, J., Gautier, P., von Kriegsheim, A., Brunsdon, H., Wheeler, A.P., Dreger, M., Houston, D.R., Dooley, C.M., Sims, A.H., Busch-Nentwich, E.M., Zon, L.I., Illingworth, R.S., Patton, E.E., PRL3-DDX21 Transcriptional Control of Endolysosomal Genes Restricts Melanocyte Stem Cell Differentiation, 317-332.e9, Copyright (2020) with permission from Elsevier. Full text @ Dev. Cell