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FIGURE 9
Chemical exposure procedure. A, Adult male and female zebrafish were placed together in a breeding tank equipped with a partition plate and a mesh tray for collecting fertilized eggs the day before spawning. B, Before chemical exposure, fertilized eggs were treated with protease type XIV for dechorionation. C, The dechorionated eggs were placed in a 6‐well plate and treated with a teratogen. D, Time course of chemical treatment. Chemical treatment started at 4 hpf and exposure medium was replaced daily. The samples were collected at 96 hpf and were processed for further analysis
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