PUBLICATION
Chemical-induced craniofacial anomalies caused by disruption of neural crest cell development in a zebrafish model
- Authors
- Liu, S., Narumi, R., Ikeda, N., Morita, O., Tasaki, J.
- ID
- ZDB-PUB-200422-173
- Date
- 2020
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 249(7): 794-815 (Journal)
- Registered Authors
- Keywords
- Disease model, Environmental factors, Neural crest cells, Neurocristopathy, Teratogen, Zebrafish
- MeSH Terms
-
- Animals
- Apoptosis
- Cartilage/drug effects
- Cartilage/embryology
- Cell Differentiation
- Chondrocytes/cytology
- Chondrocytes/drug effects
- Craniofacial Abnormalities/chemically induced
- Craniofacial Abnormalities/embryology*
- Disease Models, Animal
- Eye/drug effects
- Eye/embryology
- Female
- Gene Expression Profiling
- Gene Expression Regulation, Developmental*
- Gene Knockdown Techniques
- Gene-Environment Interaction
- Male
- Maternal Exposure
- Neural Crest/cytology*
- Neural Crest/drug effects
- Organogenesis/drug effects
- Organogenesis/genetics
- Skull
- Teratogens*
- Zebrafish/embryology*
- Zebrafish/growth & development*
- Zebrafish Proteins/genetics
- PubMed
- 32314458 Full text @ Dev. Dyn.
Citation
Liu, S., Narumi, R., Ikeda, N., Morita, O., Tasaki, J. (2020) Chemical-induced craniofacial anomalies caused by disruption of neural crest cell development in a zebrafish model. Developmental Dynamics : an official publication of the American Association of Anatomists. 249(7):794-815.
Abstract
Background Craniofacial anomalies are among the most frequent birth defects worldwide, and are thought to be caused by gene-environment interactions. Genetically manipulated zebrafish simulate human diseases and provide great advantages for investigating the etiology and pathology of craniofacial anomalies. Although substantial advances have been made in understanding genetic factors causing craniofacial disorders, limited information about the etiology by which environmental factors, such as teratogens, induce craniofacial anomalies is available in zebrafish.
Results Zebrafish embryos displayed craniofacial malformations after teratogen treatments. Further observations revealed characteristic disruption of chondrocyte number, shape and stacking. These findings suggested aberrant development of cranial neural crest (CNC) cells, which was confirmed by gene expression analysis of the CNC. Notably, these observations suggested conserved etiological pathways between zebrafish and mammals including human. Furthermore, several of these chemicals caused malformations of the eyes, otic vesicle, and/or heart, representing a phenocopy of neurocristopathy, and these chemicals altered the expression levels of the responsible genes.
Conclusions Our results demonstrate that chemical-induced craniofacial malformation is caused by aberrant development of neural crest. This study indicates that zebrafish provide a platform for investigating contributions of environmental factors as causative agents of craniofacial anomalies and neurocristopathy. This article is protected by copyright. All rights reserved.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping