ZFIN Image: Chestnut et al., 2020, Fig. 2

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Figure Caption

Fig. 2 Identification of the transcriptional signature of endocardial progenitors.

Subclustering of the EC1 population in etv2^ci32Gt+/− embryos identified a novel endocardial subpopulation (while the remaining endothelial cells are designated as EC1a). a A section of a heatmap showing marker gene expression in endocardial, endothelial (EC1a and EC2), endothelial progenitor (EPC), and cardiomyocyte populations. b A portion of the global t-SNE plot showing the endocardial subpopulation. c Heatmap for subclustering of EC1 population showing genes enriched in endocardial and the remaining endothelial (EC1a) cells. d, e t-SNE and violin plots for selected endocardial genes. f–i In situ hybridization analysis at the 20-somite stage for selected endocardial-enriched genes fn1a and drl (f, g arrows point to endocardial expression) and endocardial-excluded genes etv2 and lmo2 (bilateral expression is present in cranial endothelial progenitors but is largely absent from the endocardial cluster). Flat-mounted embryos, ventral view, anterior is to the left. The number of embryos displaying the representative phenotype out of the total number of embryos obtained from two replicate experiments is shown.

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Acknowledgments

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