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Figure 10.
F2 ENU-mutagenized zebrafish, heterozygous for multiple mutations across the entire genome were obtained from the Wellcome Sanger Institute as part of the Zebrafish Mutation Project. At QMUL, heterozygous F2 fish were incrossed to generate 30 F3 families, each containing 10–20 nonsense or essential splice site mutations and about 1500 additional exonic and intronic point mutations. F3 Families were arbitrarily numbered 1–30. Expected mutation rate and type of mutations in coding regions are specified on the right hand side.
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