Fig 4

Fig 4

A: Costaining of telomeres and Sycp1 on sycp2^+/+ (i-ii), sycp2^its/its (iii), and sycp2^-/- (iv) spermatocyte chromosomal spreads. The regions outlined with white are shown at a higher magnification at the bottom. The nuclei of sycp2^+/+ spermatocytes are at zygonema (i; telomere detected at one end of each Sycp1 filament) and pachynema (ii; telomeres detected at both ends of Sycp1 filaments). Scale bars, 5 μm. B: Colocalization of Sycp1 fragments with telomere foci stained by the telomere-targeting polyamide at their ends in sycp2^+/+, sycp2^its/its and sycp2^-/- spermatocytes. The percentages of Sycp1 filaments that did not colocalize or that colocalized at one or both ends with telomeres are shown in gray, light green and green, respectively. Each pool corresponds to the nuclei counted in S6 Fig. Error bars indicating the SD are shown only for the plus direction. A small fraction of Sycp1 ends did not colocalize with telomere foci, but two telomere foci were observed in close proximity (Fig 4A, arrowhead). These sites were possible locations at which synapsis or desynapsis was in progress. C: Numbers of telomere foci in sycp2^+/+, sycp2^its/its and sycp2^-/- spermatocytes. Telomere foci stained by the telomere-targeting polyamide were counted in nuclei with Sycp1 filaments of each genotype. Center lines show the medians; box limits indicate the 25th and 75th percentiles as determined by R software; whiskers extend 1.5 times the interquartile range from the 25th and 75th percentiles; data points are plotted as open circles. Chromosomal spreads of one (wild-type) or two (sycp2^its/its and sycp2^-/-) individual fish were used for counting. sycp2^+/+, n = 34; sycp2^its/its, n = 41; and sycp2^-/-, n = 45. *** indicates p<0.0001 (Student’s t-test).