PUBLICATION
Sycp2 is essential for synaptonemal complex assembly, early meiotic recombination and homologous pairing in zebrafish spermatocytes
- Authors
- Takemoto, K., Imai, Y., Saito, K., Kawasaki, T., Carlton, P.M., Ishiguro, K.I., Sakai, N.
- ID
- ZDB-PUB-200225-42
- Date
- 2020
- Source
- PLoS Genetics 16: e1008640 (Journal)
- Registered Authors
- Sakai, Noriyoshi
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Gene Knockout Techniques
- Homologous Recombination*
- Male
- Mutation
- Spermatocytes/metabolism*
- Synaptonemal Complex/genetics
- Synaptonemal Complex/metabolism*
- Zebrafish/physiology*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 32092049 Full text @ PLoS Genet.
Citation
Takemoto, K., Imai, Y., Saito, K., Kawasaki, T., Carlton, P.M., Ishiguro, K.I., Sakai, N. (2020) Sycp2 is essential for synaptonemal complex assembly, early meiotic recombination and homologous pairing in zebrafish spermatocytes. PLoS Genetics. 16:e1008640.
Abstract
Meiotic recombination is essential for faithful segregation of homologous chromosomes during gametogenesis. The progression of recombination is associated with dynamic changes in meiotic chromatin structures. However, whether Sycp2, a key structural component of meiotic chromatin, is required for the initiation of meiotic recombination is still unclear in vertebrates. Here, we describe that Sycp2 is required for assembly of the synaptonemal complex and early meiotic events in zebrafish spermatocytes. Our genetic screening by N-ethyl-N-nitrosourea mutagenesis revealed that ietsugu (its), a mutant zebrafish line with an aberrant splice site in the sycp2 gene, showed a defect during meiotic prophase I. The its mutation appeared to be a hypomorphic mutation compared to sycp2 knockout mutations generated by TALEN mutagenesis. Taking advantage of these sycp2 hypomorphic and knockout mutant lines, we demonstrated that Sycp2 is required for the assembly of the synaptonemal complex that is initiated in the vicinity of telomeres in wild-type zebrafish spermatocytes. Accordingly, homologous pairing, the foci of the meiotic recombinases Dmc1/Rad51 and RPA, and γH2AX signals were largely diminished in sycp2 knockout spermatocytes. Taken together, our data indicate that Sycp2 plays a critical role in not only the assembly of the synaptonemal complex, but also early meiotic recombination and homologous pairing, in vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping