Figure 1

Figure 1

Heterogeneous Expression of tcf21, tbx18, and wt1b in the Developing Zebrafish Epicardium

(A) Fluorescence of tcf21:myr-tdTomato (magenta membrane), tbx18:myr-eGFP (green membrane), and wt1b:H2B-Dendra2 (green nucleus).

(B) Workflow to establish TgBAC(tcf21:myr-tdTomato;tbx18:myr-eGFP;wt1b:H2B-Dendra2)^ox187. (C) Schematic of epicardial fluorescence patterns and cardiac regions analyzed. dsBA, distal bulbus arteriosus; prBA, proximal bulbus arteriosus; apV, arterial pole of the ventricle; and AVC, atrioventricular canal.

(D–F) Single optical sections of mRNA stainings of (D) tcf21 (cyan) and tcf21:myr-tdTomato (magenta), (E) tbx18 (magenta) and tbx18:myr-eGFP (green), and (F) wt1b (magenta) and wt1b:H2B-Dendra2 (green). (D′–F′) Cell nuclei (asterisks) in the epicardial region surrounded by endogenous mRNA and fluorophore mRNA/protein.

(G–I) Projections of the heart in triple reporter larvae at 3 dpf (G), 5 dpf (H), and 7 dpf (I).

(G′ and H′) Single optical sections from (G) and (H).

(I′) Short projection from (I).

(G″–I″ and G‴–I‴) Representative epicardial fluorescence patterns.

(J–L) Relative quantification of epicardial fluorescence patterns across the regions indicated in (C) at 3 dpf (J), 5 dpf (K), and 7 dpf (L).

Scale bars: 20 μm in (D)–(F), 5 μm in (D′)–(F′), 50 μm in (G)–(I) and (G′)–(I′), and 10 μm in (G″)–(I″) and (G‴)–(I‴). Color channels were adjusted separately for brightness and contrast. Data in (J)–(L) are represented as mean minus standard deviation. Number of embryos analyzed: 3 dpf n = 6, 5 dpf n = 10, and 7 dpf n = 6. V, ventricle; BA, bulbus arteriosus.

See also Figure S1.