PUBLICATION
Functional Heterogeneity within the Developing Zebrafish Epicardium
- Authors
- Weinberger, M., Simões, F.C., Patient, R., Sauka-Spengler, T., Riley, P.R.
- ID
- ZDB-PUB-200225-23
- Date
- 2020
- Source
- Developmental Cell 52(5): 574-590.e6 (Journal)
- Registered Authors
- Patient, Roger K., Sauka-Spengler, Tatjana
- Keywords
- RNA-seq, development, epicardium, heart, heterogeneity, single cell, zebrafish
- Datasets
- GEO:GSE121750
- MeSH Terms
-
- Animals
- Cell Lineage*
- Gene Expression Regulation, Developmental
- Pericardium/cytology*
- Pericardium/embryology
- Pericardium/metabolism
- RNA-Seq
- Single-Cell Analysis
- Transcriptome*
- Zebrafish
- PubMed
- 32084358 Full text @ Dev. Cell
Citation
Weinberger, M., Simões, F.C., Patient, R., Sauka-Spengler, T., Riley, P.R. (2020) Functional Heterogeneity within the Developing Zebrafish Epicardium. Developmental Cell. 52(5):574-590.e6.
Abstract
The epicardium is essential during cardiac development, homeostasis, and repair, and yet fundamental insights into its underlying cell biology, notably epicardium formation, lineage heterogeneity, and functional cross-talk with other cell types in the heart, are currently lacking. In this study, we investigated epicardial heterogeneity and the functional diversity of discrete epicardial subpopulations in the developing zebrafish heart. Single-cell RNA sequencing uncovered three epicardial subpopulations with specific genetic programs and distinctive spatial distribution. Perturbation of unique gene signatures uncovered specific functions associated with each subpopulation and established epicardial roles in cell adhesion, migration, and chemotaxis as a mechanism for recruitment of leukocytes into the heart. Understanding which mechanisms epicardial cells employ to establish a functional epicardium and how they communicate with other cardiovascular cell types during development will bring us closer to repairing cellular relationships that are disrupted during cardiovascular disease.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping