Figure 5.

Figure 5.

Cell count of sMNs and chx10⁺ spinal neurons in 3-dpf larval zebrafish. A, Schematic of the positions at which Z-stacks were imaged. Counting was performed over two to three somites. B, Representative pictures of targeted rostral and caudal sections, in brightfield (BF) and fluorescent (GFP) conditions for isl1:GFP and chx10:GFP fish in wholemount preparations, and for isl1:GFP fish in sagittal sections of the spinal cord. Scale bars represent 100 μm for wholemount images and 50 μm for section images. C, Count of GFP⁺ cells for the rostral and caudal section in chx10:GFP fish. D, Count of GFP⁺ cells for the rostral and caudal section in isl1:GFP fish. Counts in wholemount and sectioned fish were pooled together. E, Count of zn-8⁺ cells for the rostral and caudal section in isl1:GFP fish. Two-tailed paired Student’s t test (GFP⁺ in chx10:GFP rostral-caudal, p = 0.3196; GFP⁺ in isl1:GFP rostral-caudal, p = 0.0231; zn-8⁺ in isl1:GFP rostral-caudal, p = 0.7274); *p < 0.05, indicating significance. Gray circles are the average of three to four independent counts for each fish. Black circles are the average across all fish. Error bars display SD.