ZFIN Image: Wang et al., 2019, Fig 3

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Figure Caption

Fig 3

miR-1 morphants displayed defective migration of neural crest cells. Live embryo imaging in miR-1 morphants and negative control Tg (sox10: EGFP) embryos was used to analyze the movement of neural crest cells, viewed dorsally. (A-D) Representative images are shown from the time-lapse photography from 6 segment stage (ss) to 20 ss. At 6ss, 10 ss, 16 ss and 20 ss, neural crest cells in miR-1 morphants displayed ectopic EGFP expression (red arrowheads) in the dorsal midline (A, B, C and D). At 24 hpf, expression of snai1b (E) and tfap2a (F) in negative control and miR-1 morphants is shown. At this time, increasing numbers of the migrating lateral line primordium (arrow) expressed tfap2a and snail1b. The expression of neural crest migration markers (snai1b, tfap2a) was reduced (arrows) (E, F). microRNA-1, miR-1; morpholino, MO; ss, segment stage.

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