Fig. 5

Fig. 5

Effects of Parkin on ER–mitochondria contacts. Immunofluorescence against mitochondria (Tom20, cyan) is shown in the panels on the middle. The green channel is the merge of several planes. Scale bar 20 µm. a Representative confocal pictures of HeLa cells expressing the SPLICS_S probe along with Parkin-2A-mCherry-CAAX (bottom panels). b Quantification of SPLICS_S contacts by 3D rendering of complete z-stacks. Mean ± SEM: Ctrl 61 ± 3, n = 57 cells; Parkin 94 ± 6, n = 33 cells; Parkin CCCP 65 ± 7, n = 26 cells. c Representative confocal pictures of HeLa cells expressing the SPLICS_L probe along with Parkin-2A-mCherry-CAAX (bottom panels). d Quantification of the SPLICS_L contacts by 3D rendering of complete z-stacks. Mean ± SEM: CTRL 227 ± 9, n = 44 cells; Parkin 193 ± 11, n = 32 cells; Parkin CCCP 159 ± 8, n = 22 cells. Data shown are the result of 3–8 independent experiments. *p ≤ 0.05, **p ≤ 0.01, ****p ≤ 0.0001.