IMAGE

Fig 2

ID

ZDB-IMAGE-191230-1465

Genes

Antibodies

Ab13-smad

Publication

Ye et al., 2019 - Marcksb plays a key role in the secretory pathway of zebrafish Bmp2b

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Figures for Ye et al., 2019

Image

Figure Caption

Fig 2 Marcksb is required for activation of BMP signaling.

(A) WISH analysis showed that the transcriptional levels of downstream targets of BMP signaling—szl and ved were decreased in marcksb morphants. (B) The percentage of embryos with normal or decreased expression. “n” represents the number of embryos we observed. (C) The overall intensity of p-Smad1/5/9 at ventral region in marcksb morphants was dramatically decreased when compared with wildtype. (D) The normalized fluorescent intensity of P-Smad1/5/9. Error bars of light blue and light red show S.E.M. (E) Knockdown of marcksb could partially rescue the ventralization phenotype caused by injection of bmp2b mRNA (5 pg bmp2b mRNA per embryo). The statistical data are shown in the bar graphs with the number of observed embryos. “n” represents the number of embryos we observed. (F) Overexpression of bmp2b caused dramatic dorsal expansion of szl and ved expression while knockdown of marcksb in the bmp2b-overexpressed embryos partially restored their expression patterns. (G) The percentage of embryos with robust-increased or dorsal-inhibited expression. “n” represents the number of embryos we observed.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.