Fig. 2

Glial bridge formation and motor neurons regeneration after SCI in 5 dpf larvae. Labeling pattern of Hb9:GFP transgenic larvae with double immunohistochemistry against GFAP (red) to reveal the glial bridge (a–e) and against GFP (green) to reveal HB9⁺ motor neurons (a’–e’) from 1 to 6 dpi. (a”–e”) Merged channels for each time point. White arrowheads show the lesion site and asterisks highlight the regenerating HB9⁺ peripheral axons. Rostral side is to the left and dorsal side is up. Number of HB9⁺ motor neurons (f) and length of HB9⁺ peripheral axons (g) at the lesion site from 1 to 6 dpi. SCI_spinal cord injury, dpf_days-post-fertilization, hpi_hours-post-injury Mean ± s.e.m. of one experiment is presented. ns - not significant, *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001, Student’s t-test with Welch’s correction. Scale bar: 100 µm.