ZFIN Image: Liu et al., 2019, Fig. 3

Image

Figure Caption

Fig. 3

Loss of cilia genes induces hematopoietic stem and progenitor cell (HSPC) defects. a Whole-mount in situ hybridization (WISH) results of HSPC markers (runx1 and cmyb) in the aorta-gonad-mesonephros (AGM) region in control and fsd1 morphants at 36 hpf. The red arrowheads indicate the expression of HSPC markers runx1 and cmyb in DA. b Western blotting showing the protein level of Runx1 in control and fsd1 morphants at 26 hpf. c WISH analysis showing the expression of HSPC markers runx1 and cmyb (red arrowheads) in the DA in fsd1^−/−. d Western blotting showing the protein level of Runx1 in fsd1^−/−. e Representative images showing runx1 and cmyb expression in control, pkd2, kif3a, and ift88 morphants at 33 hpf. The red arrowheads indicate the expression of HSPC markers runx1 and cmyb. f WISH analysis showing the expression of HSPC marker runx1 in the DA in pkd2^−/− or ift88^−/− with a sub-effective dose of pkd2 MO (0.5 ng per embryo, pkd2 MO_low) or ift88 MO (0.8 ng per embryo, ift88 MO_low). The red arrowheads indicate the expression of HSPC marker runx1. Scale bars, 100 µm

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.