PUBLICATION
Primary cilia regulate hematopoietic stem and progenitor cell specification through Notch signaling in zebrafish
- Authors
- Liu, Z., Tu, H., Kang, Y., Xue, Y., Ma, D., Zhao, C., Li, H., Wang, L., Liu, F.
- ID
- ZDB-PUB-190425-9
- Date
- 2019
- Source
- Nature communications 10: 1839 (Journal)
- Registered Authors
- Liu, Feng, Liu, Zhibin, Ma, Dongyuan, Wang, Lu, Zhao, Chengtian
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Cilia/genetics
- Cilia/metabolism*
- Embryo, Nonmammalian
- Embryonic Development/physiology
- Hemangioblasts/cytology
- Hemangioblasts/metabolism
- Hematopoiesis/physiology
- Hematopoietic Stem Cells/physiology*
- Models, Animal
- Receptors, Notch/metabolism*
- Signal Transduction/physiology*
- Zebrafish/physiology
- Zebrafish Proteins/metabolism*
- PubMed
- 31015398 Full text @ Nat. Commun.
Citation
Liu, Z., Tu, H., Kang, Y., Xue, Y., Ma, D., Zhao, C., Li, H., Wang, L., Liu, F. (2019) Primary cilia regulate hematopoietic stem and progenitor cell specification through Notch signaling in zebrafish. Nature communications. 10:1839.
Abstract
Hematopoietic stem and progenitor cells (HSPCs) are capable of producing all mature blood lineages, as well as maintaining the self-renewal ability throughout life. The hairy-like organelle, cilium, is present in most types of vertebrate cells, and plays important roles in various biological processes. However, it is unclear whether and how cilia regulate HSPC development in vertebrates. Here, we show that cilia-specific genes, involved in primary cilia formation and function, are required for HSPC development, especially in hemogenic endothelium (HE) specification in zebrafish embryos. Blocking primary cilia formation or function by genetic or chemical manipulations impairs HSPC development. Mechanistically, we uncover that primary cilia in endothelial cells transduce Notch signal to the earliest HE for proper HSPC specification during embryogenesis. Altogether, our findings reveal a pivotal role of endothelial primary cilia in HSPC development, and may shed lights into in vitro directed differentiation of HSPCs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping