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Fig. 2
JAM-A indicated B-lymphoma cell stemness. (A) As assessed by Whole-mount in Situ Hybridization and real-time quantitative RT-PCR, c-myb and runx1 were overexpressed after 26 h microinjecting jam-1 mRNA (jam-1) in Zebrafish embryos. WT, wild type. (B,C) B-lymphoma cell line DB and SU-DHL-4 were transfected with control vector (Vector) and JAM-A vector (JAM-A). Ectopic expression of JAM-A was associated with increased colony formation (B) and stem-cell marker CD133 and CD34 expression (C). (D) DLBCL patients with high JAM-A expression displayed increased CD133 positivity. (E) Patients in high JAM-A expression group were enriched for a stem cell gene signature, as revealed by RNA sequencing. Data in (A), (B) and (C) are representative of three independent experiments.