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Fig. 4
There Is a Negative Feedback Loop between mespa/mespb and ripply1 Genes
(A) Embryos from different genetic backgrounds were fixed immediately or 30 min of recovery after 60-min heat shock at 37°C.
(B and C) ISH hybridization pictures of ripply1 gene in (B) hsp70l:mespab-myc or (C) hsp70l:mespbb-myc embryos after a 1-h heat shock. These experiments were repeated three times, and 104–96 embryos were evaluated for each genotype, respectively.
(D and E) ISH hybridization pictures of (D) mespba and (E) mespaa in hsp70l:ripply1-myc embryos after a 1-h heat shock. These experiments were repeated twice and 45–47 embryos were evaluated for all probes.
(F) Regulatory network among scoliosis-linked genes. herrepresents both her1 and her7. delta/notch represents the transcriptional activation of its ligand DeltaC and subsequent pathway activation. Thicker arrows indicate stronger and differential dependence of transcription of each mesp gene on two different transcription factors. Red color represents regulatory interactions inferred from time-controlled perturbation experiments in this study. In the computational model, transcription, translation, protein and mRNA degradation, formation of protein complexes, and proteolytic Notch activation are included for the nodes in the network. WT, wild-type.