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Fig. 3
Tcf7l1a cell autonomously promotes rx3 expression in the eye field.
(A–F) Dorsal views of confocal images of rx3 mRNA expression (red) detected by fluorescent in situ hybridisation at 10hpf in the anterior neural plates of chimeric embryos containing transplants of (A–C) wildtype (GFP+) donor cells in MZtcf7l1a-/- host embryos (100%, n = 13), and (D–F) MZtcf7l1a-/- (GFP+) donor cells in wildtype host embryos (100%, n = 9). Dotted line outlines eye fields; note in A-C that rx3 expression extends considerably caudal to the reduced mutant eye field on the side of the neural plate containing wild-type cells. Dashed line marks the embryo midline. (G–J) In situ hybridisation of rx3 and pax2a in sibling (G, H) and Ztcf7l1a-/- (I, J) 9hpf embryos, uninjected (G, I) or injected with 50 pg of dkk1 mRNA (H, J). Abbreviations; EF, eyefield; mb, midbrain Scale Bars = 200 µm.