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Fig. 4 No axonal translation occurs with soma-restricted 3′UTR sequences. Representative examples of sites of protein synthesis revealed by HA/Venus immunostaining of Danoprevir-treated, SPoT transgenic embryos containing 3'UTRs with restricted axonal mRNA localization. (A–C) Superimposed Venus (green) and HA (magenta) immunostaining signals. (A′–C′) HA immunostaining signal intensity color-coded with imageJ ‘Fire’ lookup table. Color-intensity correspondence is represented in the calibration bar. (A,B) Transgenic line, Tg(SPoT_neuroD). Danoprevir treatment time=1.5 h. (C) Transgenic line, Tg(SPoT_chicken ß-actin-ΔZipcode). Danoprevir treatment time, 1.5 h. Scale bars: 20 µm.