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Fig. 8 Microtubule filament stability is required for JNK-mediated basal anisotropic cell shape. (A-D) Digital slices from z-series images of memGFP-injected wild-type embryos treated at 16 ss with DMSO (A,B) or SP600125 (C,D). Embryos were then treated at 18 ss with DMSO (A,C) or paclitaxel (B,D) and imaged at 24 ss. MHBC cells are outlined in yellow. (E) Quantification of anisotropic cell shape. All basal x:z ratios were compared post-hoc with the SP600125/DMSO treatment. Boxplots indicate the 25th and 75th percentiles and the median. Four independent experiments are represented. DMSO/DMSO n=6; DMSO/paclitaxel n=6; SP600125/DMSO n=10; SP600125/paclitaxel n=7. *P<0.05, ***P<0.005. Scale bars: 10 μm.