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Fig. 4
irx3b depletion converts the DE segment to CS fate.
(A) Panels show lateral views of 18 hpf, 20 hpf, 22 hpf and 24 hpf wild-type and irx3b morphant embryos co-stained for cdh17 (red) and slc12a1 (purple). Black arrowheads highlight the initiation of pronephric phenotypes in irx3b morphants. (B) Panels show lateral views of control (un-injected) embryos and irx3b morphants double-stained for either slc12a1(red)/stc1(purple), cdh17(red)/stc1(purple) or cdh17(red)/slc12a1(purple) at 24 hpf, 36 hpf and 50 hpf.
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