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Fig. 3

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ZDB-IMAGE-181207-59
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Figures for Teng et al., 2018
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Fig. 3

Expression of tcf12 and twist1b in multiple sutures of zebrafish.

(A) Schematic of the zebrafish skull depicting positions of sections (dotted lines) used for RNAscope in situ hybridizations. cs, coronal suture; ms, metopic suture; ss, sagittal suture; fb, frontal bone; pb, parietal bone. (B–D) In situ hybridizations on sections taken from zebrafish at 14 mm standard length. Red puncta indicate positive expression. DapB (B) was included as a negative control, with suture mesenchyme outlined in a dashed white line for reference. Expression of tcf12 (C) and twist1b (D) was detected in the metopic, coronal and sagittal suture mesenchyme, with tcf12 also expressed broadly outside the sutures. n = 3 for each experiment. Scale bar, 50 µm.

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