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Fig. 6
M. circinelloides promotes neutrophils and macrophages depletion in the head kidney. (A) Representative sections of zebrafish head kidneys infected with 2.5 × 105 R7B spores/fish at 16 hpi and immunostained with anti-Lcp1 (pan-leukocyte marker) and anti-Mpx (neutrophil marker) antibodies. Note the robust depletion of myeloid cells. Scale bars 20 µm. (B) Quantification of Lcp1+ and Mpx+ cells is shown as the mean ± SEM of the immunostained area/total area of 4 randomly distributed optical areas from 4 fish at ×200 magnification. *P<0.05; **P<0.01; ***P<0.001.
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