IMAGE

Fig. 6

ID
ZDB-IMAGE-180918-27
Genes
Antibodies
Source
Figures for Xing et al., 2018
Image
Figure Caption

Fig. 6

Knockdown of other dvl genes has no effect in MZdvl2;MZdvl3a mutants.

The embryos were injected with coMO (2.5 ng) or a mixture of equal amount of dvl1a/1b/3bMOs (6 ng in total) at 1-cell stage, except for immunostaining. All mutant embryos were imaged after different analyses, followed by genotyping. (A-D) In situ hybridization analysis of goosecoid (gsc) expression in indicated embryos at dome stage. Animal pole view with dorsal region on the right. (E) Endogenous ß-catenin nuclear accumulation (arrows) in dorsal marginal cells of a WT embryo at high stage. (F-I) In situ hybridization analysis of chordin expression in indicated embryos at dome stage. Animal pole view with dorsal region on the right. (J) Endogenous ß-catenin nuclear accumulation (arrows) in dorsal marginal cells of an MZdvl2;MZdvl3a mutant at high stage. (K-O) Phenotypes of indicated embryos at 11.5 hpf. Lateral view, with a dvl1a/1b/3bMOs-injected MZdvl2;MZdvl3a embryo also shown in dorsal view (O). (P-S) Phenotypes of indicated embryos at 30 hpf. Lateral view, note that injection of dvl1a/1b/3bMOs does not change the phenotype of WT and MZdvl2;MZdvl3a embryos. (T) Statistical analysis of the extent of axis extension delay in indicated embryos at 11.5 hpf. Bars represent the mean ± s.d. from indicated numbers of embryos (NS, not significant). Scale bars: (A-D, F-I) 400 μm; (E, J) 25 μm; (K-S) 400 μm.

Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.