Fig. 2

Fig. 2

Mutations in dock1 cause decreased mbp expression in the PNS. a-d) Lateral views of mbp expression by WISH at 3 dpf. Arrowheads indicate PLLn. Asterisks indicates the CNS. Inset panels show a magnified view of PLLn. Scale bars?=?100 ?m. a) Control larvae robustly express mbp in the PLLn (n?=?29/38). b) dock1^stl145 homozygous mutants exhibit strongly reduced mbp expression in the PLLn (n?=?6/6). c) Control larvae injected with dock1 mRNA exhibit strong expression of mbp in the PLLn (n?=?50/53). d) dock1^stl145 homozygous mutants injected with dock1 mRNA robustly express mbp in the PLLn (n?=?13/25). e) Quantification of the percent phenotypic classes larvae were scored for mbp expression in the PLLn at 3 dpf. Control?=?pooled uninjected and phenol red injected larvae. f) A schematic of the Dock1 protein with the locations of the stl366, stl365, and stl145 lesions indicated. g-j) Lateral views of mbp expression by WISH at 3 dpf. Arrowheads indicate the PLLn. Asterisks indicate the CNS. Inset panels show a magnified view of PLLn. Scale bars?=?100 ?m. g) dock1^stl365 homozygous mutants (n?=?20) and h) dock1^stl366 homozygous mutants exhibit reduced mbp expression in the PLLn (n?=?15/16). i) dock1^{stl145/stl365} compound heterozygotes and j) dock1^{stl145/stl366} compound heterozygotes exhibit reduced mbp expression in the PLLn. k, l) Quantification of WISH for mbp from dock1^stl365 (k) and dock1^stl366 (l) in-crosses based on phenotypic classes and genotypes for the respective lesions. * p?<?0.05, *** p?<?0.001, **** p?<?0.0001, Chi-squared analysis