ZFIN Image: Pinto et al., 2018, Fig. 4

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Figure Caption

Fig. 4

Characterisation of dmrt2a overexpression phenotype: somite differentiation and formation markers. a Somite differentiation gene pcdh8 (8-somite stage). b Somite boundary gene mespb (8-somite stage). c Muscle differentiation marker myod1 (14-somite stage). d Somite differentiation gene cb1045 (24 hpf). The embryos shown illustrate the different phenotypes obtained. e F-actin staining in control (n = 40/42) and in Tg(hsp70:HA-dmrt2a) after dmrt2a overexpression (n = 22/51) where lack of intersomitic borders is observed (i, ii). Embryos between 8 and 10-somite stage were used. Sym: symmetric, Asym: asymmetric, BD: border defect, hpf: hours post-fertilisation. Scale bar: 45 μm (e) and 20 μm (zoomed panels i and ii)

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Acknowledgments

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