Fig. 6

Fig. 6

Neural specification and neural differentiation are impaired in fbl^hi2581Tg mutant embryos. (A) RT-qPCR quantification of the relative levels of neuroD1 mRNA. Purple: wild-type, gray: siblings, pink: mutants. (B) Expression patterns of eomes and otx2, two markers of neural specification, in 3 dpf wild-type (B1, B3) and fbl^hi2581Tg mutant embryos (B2, B4). The expression of eomes(B1-B2), a marker of forebrain specification, was similar in wild-type and mutant embryos, whereas that of otx2(B3-B4), a marker of midbrain specification, is absent in fbl^hi2581Tg mutant embryos. Scale bars: 50 µm. Hb: hindbrain, OT: optic tectum, Tel: telencephalon. (C) Horizontal optical sections of elavl3 (marker of neural differentiation) labeling in 2dpf wild-type (C1-C3) and mutant (C4-C6) embryos and in 3dpf wild-type (D1-D3) and mutant (D4-D6) embryos. Gray: DAPI staining, pink: Elavl3 staining. Scale bars: 50 µm. Anterior is to the left. (E) Volume rendering of the DiI-positive domains (gray) and surface rendering of a manual segmentation of the Elavl3-positive (green) domains in 3dpf wild-type (E1), fbl siblings (E2) and fbl^hi2381 mutant embryos (E3). For lateral views, anterior to the left and dorsal to the top. White arrows point out to the midbrain. Neural differentiation is specifically impaired dorsally in fbl mutant.