ZFIN Image: Owlarn et al., 2017, Fig. 6

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Figure Caption

Fig. 6

The wound epidermis of dormant fins is not a barrier to regeneration. a Transient inhibition of Wnt/β-catenin signaling using overexpression of Axin1 in hsp70l:Axin1-YFP transgenic fish resulted in a stable blockage of fin ray regeneration. n = 9 fish. b Amputation and heat-shock regime used to produce recessed rays in dormant fins. Note that interray skin healed distally to non-regenerating recessed rays (asterisk) despite Axin1-YFP expression. c Skin wounding applied to the skin distal to recessed dormant rays (black arrowheads) induced regenerative growth beyond the primary amputation plane. Asterisks indicate non-injured control rays. Graph depicts the fraction of regenerating rays. ***p < 0.001 χ ² test. n (non-injured) = 48 rays, 48 fish, two experiments, n (wounded) = 69 rays, 50 fish, two experiments. Scale bars: 500 µm

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