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Fig. 1
Characterization of ?heartless?. (A). Schematic description of the ?heartless? model and the experimental strategy. (B). Specific absence of cardiomyocytes in ?heartless?. Scale bars: 1?mm (top); 1?mm (middle); 100?µm (bottom). Red, cardiomyocytes; green, vECs and endocardium. Bright-field/DIC images are shown for WT-MTZ as they do not carry a fluorescent reporter. (C). qRT-PCR analysis of pan-hypoxia indicator genes in ?heartless?. ***P<0.001; n.s., not significant. n=8 for both Tg(cmlc2:mcherry-NTR)+DMSO and ?heartless?. (D). Volcano plot representing the differentially expressed genes in ?heartless?, as compared to Tg(cmlc2:mcherry-NTR)+DMSO (NTR+DMSO) (left) and WT+MTZ (right). n=2. Up- and downregulated genes (as also confirmed by qRT-PCR, see Table S1) are highlighted in red and blue, respectively. (E). Organ-specific expression patterns of differentially expressed genes in ?heartless?. They are placed in each organ. Up- and downregulated genes are indicated by ?and ?, respectively. Some of the genes unaffected in ?heartless? are also included and indicated by ?. Many of the liver-specific genes were also confirmed by whole-body transcriptome analysis of the liver-ablated larvae (?liverless?) (Fig. S3).