Fig. 2
Spatiotemporal expression pattern of DYRK1A transcript in zebrafish embryos. (a) Location of anti-sense RNA probe for detection of zebrafish DYRK1A using WISH. (b) Detection by Probe 1 at indicated stages. (c) Detection by Probe 2 at indicated stages. Arrows show the stronger expression region of DYRK1A. The same expression pattern of DYRK1A shown by two non-overlapping probes, demonstrating the specificity and identical spatially restricted expression patterns of DYRK1A in zebrafish early embryogenesis. Embryo orientations: 2-cell and Sphere stage, lateral views with the animal pole oriented at the top; Shield stage, lateral view with the dorsal side oriented at the right; 8-somite, Prim-5 and Prim-25 stage, lateral views with anterior oriented toward the left.