ZFIN Image: Du et al., 2014, Fig. S3

Image

Figure Caption

Fig. S3

Setdb2 or Dvr1 knockdown and overexpression of dvr1 induce defects of cell polarity at the tail-bud stage. (A–D) Representative confocal images of notochord cells in control embryos (n=4 embryos, 80 cells), setdb2 morphants (n=4 embryos, 80 cells), dvr1 morphants (mo^ATG) (n=4 embryos, 80 cells) and dvr1 mRNA overexpression embryos (n=4 embryos, 80 cells) co-injected at the one-cell stage with 100 pg mRNA coding for membrane-targeted RFP. Anterior is upwards. The boundaries of the notochord are marked with dotted white lines. (E-F) Length-width ratio (LWR) of notochord cells and mediolateral alignment (ML alignment) at the tail-bud stage were measured with ImageJ2X. The rose diagrams were drawn using Rose.NET. Scale bar: 20 μm. The statistical significance of difference is indicated by asterisks (^**, P<0.01; ^****, P<0.0001).

Acknowledgments

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Reprinted from Developmental Biology, 392(2), Du, T.T., Xu, P.F., Dong, Z.W., Fan, H.B., Jin, Y., Dong, M., Chen, Y., Pan, W.J., Ren, R.B., Liu, T.X., Deng, M., Huang, Q.H., Setdb2 controls convergence and extension movements during zebrafish gastrulation by transcriptional regulation of dvr1, 233-44, Copyright (2014) with permission from Elsevier. Full text @ Dev. Biol.