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Fig. 4
Independent trafficking of Reck and Adgra2 to the plasma membrane. (A) Single-plane confocal images of non-permeabilized HEK293T cells 48?h after transfection with HA-reck and adgra2-EGFP variants, as indicated. (B) Schematic representation of the genetic lesions of ADGRA2?/? and RECK?/? cells. The position of the frame-shift mutation is indicated by the red line. See Fig. 1A for schematic labels. (C,D) Single-plane confocal images of non-permeabilized ADGRA2?/? and RECK?/? HEK293T cells 48?h after transfection with adgra2-EGFP (C) and HA-reck (D) constructs. In all panels, EGFP is detected by direct fluorescence and the HA-Reck fusion by anti-HA indirect immunofluorescence. Cells were additionally transfected with a Wnt7a (mouse gene) expression construct. Nuclei were counterstained with Hoechst. Scale bars: 10??m.