ZFIN Image: Pfefferli et al., 2017, Fig. 2

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Figure Caption

Fig. 2

ctgfa^careg:EGFP does not reproduce endogenous ctgfa expression in peri-injury tissues.

(a–h) In situ hybridization using ctgfa and enhanced green fluorescent protein (egfp) probes on sections of hearts at 14 d.p.ci. and fins at 3 d.p.a. of ctgfa^careg:EGFP transgenic fish shows distinct expression patterns. (a,b) ctgfa mRNA is not detected in the myocardium, but in a subset of non-myocytes in the post-infarcted tissue. (c,d) egfp mRNA is expressed in the outer wall of the myocardium and at the peri-injury zone (yellow arrowheads). (e,f) ctgfa mRNA is present in a few bone-associated cells of the fin, while (g,h) egfp mRNA is detected in the blastema. N=6. (i–m) careg:EGFP and careg:dmKO2 double transgenic fish display an overlapping expression pattern in the regenerating heart at 7 d.p.ci. and the fin at 1 d.p.a. N=4.

Figure Data

Expression / Labeling details

Acknowledgments

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