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Fig. 4
FL-DSLM volume of α-actin:GFP zebrafish muscle collected with 200 ms exposure at 1 μm intervals. Maximum intensity projections of stack in the xy (left) and yz (right) planes for (A) the intensity and (B) the lifetime. The short lifetime of the autofluorescence on the skin can be distinguished from the long lifetime GFP (purple) expressed in the muscle. Green intensity has been artificially brightened slightly to highlight the thin skin layer against the bright EGFP signal. The scale bar is 50 μm.
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Full text @ Opt. Lett.