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Fig. S1 D
NgAgo based lama1 gene knockdown caused notochord defects phenotype in zebrafish. (A) Microscopy analysis of notochord in control and NgAgo-2nls and lama1 FW-guide DNA 1 coinjected embryos, lateral view. Red arrowhead indicates notochord. (B) Relative mRNA levels of zebrafish lama1 in 30 hpf control and lama1 FW-guide DNA 1 and NgAgo-2nls mRNA coinjected embryos. T-test; ****, P<0.0001. (C) Statistical analysis of phenotype efficiency in control and NgAgo-2nls and lama1 FW-guide DNA 1 coinjected embryos. χ² test; ****, P<0.0001.
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